10355 1 ap (Proteintech)

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Proteintech 10355 1 ap
The information on the antibody.

10355 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1421 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/28248+1+ap/P21+Antibody/pmc13621234-1-4-8
Average 96 stars, based on 1421 article reviews

10355 1 ap - by Bioz Stars, 2026-10

96/100 stars

Images

1) Product Images from "Ghrelin Alleviates Aging‐Related Cognitive Impairment by Regulating Autophagy‐Related Signaling via the SHBG / JNK1 /Beclin1 Axis in Microglia"

Article Title: Ghrelin Alleviates Aging‐Related Cognitive Impairment by Regulating Autophagy‐Related Signaling via the SHBG / JNK1 /Beclin1 Axis in Microglia

Journal: Aging Cell

doi: 10.1111/acel.70732

Figure Legend Snippet: The information on the antibody.

Techniques Used:

Incubation:

Article Title: Spatio-Temporal Characterization of Cellular Senescence Hallmarks in Experimental Ischemic Stroke.
Article Snippet: Cryoprotected brains were flash-frozen on dry ice, mounted in Tissue-Tek OCT compound (Sakura Finetek, Torrance, CA, USA), cryostat-cut into 20 μm thick coronal sections (0.2 to −1.8 mm from bregma), and stored at −20 ◦C. .. Selected slices were incubated with sodium borohydride for 30 min, blocked with 10% normal goat serum (#S-1000-20, Vector Laboratories, Newark, CA, USA) in PBS with 0.8% Triton X-100 for 1 h at room temperature, and then incubated overnight at 4 ◦C with the following primary antibodies: (1) anti-p16 ([1:250], sc-1661, Santa Cruz Biotechnology, Dallas, TX, USA); (2) anti-p21 ([1:250], 28248-1-AP, Proteintech, Rosemont, IL, USA); (3) anti-LB1 ([1:100], sc-374015, Santa Cruz Biotechnology, Dallas, TX, USA); (4) anti-NeuN ([1:250], mAb #12943, Cell Signaling, Danvers, MA, USA); (5) anti-GFAP ([1:500], #840001, Biolegend, San Diego, CA, USA); and (6) anti-Iba1 ([1:500], #019-19741, Fujifilm Wako Pure Chemical Corporation, Chuo-ku, Osaka, Japan). .. Afterward, the sections were incubated for 1 h at room temperature with the corresponding secondary antibodies: (1) Alexa Fluor 594 goat anti-mouse IgG ([1:200], #A11005, Invitrogen, Waltham, MA, USA); (2) goat anti-rabbit IgG, DyLight 488 ([1:200], # DI-1488-1.5, Vector Laboratories, Newark, CA, USA); or (3) Alexa Fluor 568 goat anti-rabbit IgG ([1:200], #A11036, Invitrogen, Waltham, MA, USA), followed by nuclear staining with DAPI (#A1001, ITW Reagents, Milan, Italy) and mounting with FluorSave (#345789, Millipore, Darmstadt, Germany).

Article Title: Spatio-Temporal Characterization of Cellular Senescence Hallmarks in Experimental Ischemic Stroke
Article Snippet: Cryoprotected brains were flash-frozen on dry ice, mounted in Tissue-Tek OCT compound (Sakura Finetek, Torrance, CA, USA), cryostat-cut into 20 μm thick coronal sections (0.2 to −1.8 mm from bregma), and stored at −20 °C. .. Selected slices were incubated with sodium borohydride for 30 min, blocked with 10% normal goat serum (#S-1000-20, Vector Laboratories, Newark, CA, USA) in PBS with 0.8% Triton X-100 for 1 h at room temperature, and then incubated overnight at 4 °C with the following primary antibodies: (1) anti-p16 ([1:250], sc-1661, Santa Cruz Biotechnology, Dallas, TX, USA); (2) anti-p21 ([1:250], 28248-1-AP, Proteintech, Rosemont, IL, USA); (3) anti-LB1 ([1:100], sc-374015, Santa Cruz Biotechnology, Dallas, TX, USA); (4) anti-NeuN ([1:250], mAb #12943, Cell Signaling, Danvers, MA, USA); (5) anti-GFAP ([1:500], #840001, Biolegend, San Diego, CA, USA); and (6) anti-Iba1 ([1:500], #019-19741, Fujifilm Wako Pure Chemical Corporation, Chuo-ku, Osaka, Japan). .. Afterward, the sections were incubated for 1 h at room temperature with the corresponding secondary antibodies: (1) Alexa Fluor 594 goat anti-mouse IgG ([1:200], #A11005, Invitrogen, Waltham, MA, USA); (2) goat anti-rabbit IgG, DyLight 488 ([1:200], # DI-1488-1.5, Vector Laboratories, Newark, CA, USA); or (3) Alexa Fluor 568 goat anti-rabbit IgG ([1:200], #A11036, Invitrogen, Waltham, MA, USA), followed by nuclear staining with DAPI (#A1001, ITW Reagents, Milan, Italy) and mounting with FluorSave (#345789, Millipore, Darmstadt, Germany).

Article Title: PFPeA exposure drives hepatoxicity and liver fibrosis via oxidative stress/Wnt5a-induced hepatocyte senescence.
Article Snippet: After gel electrophoresis using 12 % SDSPAGE, protein was transferred onto polyvinylidene fluoride membranes (Millipore, USA). .. The membranes were blocked by NcmBlot blocking buffer for 0.5 h (New Cell&Molecular Biotech, P30500, China), then incubated in the primary antibodies over night at 4 °C (γH2AX, Cat. 9718S, CST, USA, 1:2500; p16, Cat. 10883–1-AP, Proteintech, China, 1:3000, 28416–1-AP, Proteintech, China, 1:2000; p21, Cat. 10355–1- AP, Proteintech, China, 1:5000, 28248–1-AP, Proteintech, China, 1:2000; IL-6, Cat. 83795–7-RR, Proteintech, China, 1:5000, 21865–1- AP, Proteintech, China, 1:1000; COL1A1, Cat. 66761–1-Ig, Proteintech, China, 1:2500; α-SMA, Cat. 14395–1-AP, Proteintech, China, 1:2500; α-tubulin, Cat. 11224–1-AP, Proteintech, China, 1:5000). .. Following incubation with the HRP-conjugated secondary antibody (Cat. SA00001–2, SA00001–1, Proteintech, China, 1:10000) for 1 h at room temperature, an ECL kit (New Cell&Molecular Biotech; P2300, China) was used to visualize the immunocomplex via Image Lab software (Biorad, USA) and quantified by Image J software.

Article Title: Zuogui pills ameliorate chemotherapy-induced ovarian aging by improving stemness, regulating cell cycle and reducing apoptosis of oogonial stem cells via the Notch1/Nrf2 pathway.
Article Snippet: Ethnopharmacological relevance: Zuogui Pills (ZGP) is a classic traditional Chinese herbal formula originating from the Ming Dynasty.. It has been widely used in the treatment of kidney deficiency-related diseases, including

Article Title: Aristolochic acid-induced DNA adduct formation triggers acute DNA damage response in rat kidney proximal tubular cells.
Article Snippet: Aristolochic acid nephropathy (AAN) is a form of acute kidney injury triggered by the ingestion of aristolochic acid (AA), characterized by significant degeneration and loss of cells in the proximal tubules.. Previous reports of AA-induced acute kidney injury have reported that AA-induced cytotoxicity can occur within a short period, up to 24 h; however, there are few reports on the relationship between AA-DNA adduct formation and cytotoxic mechanism during the acute phase.. In this study, we aimed to elucidate the toxicological mechanisms in the initial phase of AA exposure by examining the effects of AA on NRK-52E rat proximal tubular cells within 24 h. We detected the formation of AA-DNA adducts as early as 4 h post-exposure, indicating that 50 μM of AA causes DNA damage.

Western Blot:

Article Title: PRMT5 Regulates Senescence in Retinal Ganglion Cells by Targeting the Wnt/β-Catenin Signaling Cascade
Article Snippet: Rabbit anti–γ-H2AX , 1:1000 (WB)/1:250 (IF) , Cell Signaling Technology , #9718. .. Rabbit anti-p21 CIP1 , 1:1000 (WB) , Proteintech , 28248-1-AP. .. Rabbit anti-p16 INK4a , 1:1000 (WB)/1:100 (IF) , Abcam , ab211542.

Blocking Assay:

other:

Article Title: Inhibition of the JAK2/STAT3 Pathway Attenuates D‐Galactose‐Induced Nucleus Pulposus Cell Senescence and Intervertebral Disc Degeneration
Article Snippet: p21 , Proteintech , 28248‐1‐AP , 18 , 1:400.

Immunohistochemistry:

Article Title: Impaired hemoglobin clearance by sinusoidal endothelium promotes vaso-occlusion and liver injury in sickle cell disease.
Article Snippet: .. Primary Antibody for IHC/IF Name Concentration Company Orig concentration CD31 1:100 dilution Dianova DIA-310 0,2 mg/mL P21 (new) 1:100 dilution Proteintech 28248-1-AP 500 μg/mL P53 1:100 dilution Cell Signaling Technology 2524T lyve1 1:100 dilution ReliaTech GmbH 103-PA50 1.0 mg/mL. .. Hb-AF647 1:100 dilution Biomagnis lot# C101006 0.85 mg/mL MFG8 1:100 dilution Invitrogen PA5-109955 1.65 mg/mL CLEF4C 1:100 dilution R&D Systems AF2784 0.2 mg/mL CD11b 1:100 dilution Novus Biologicals NB11089474SS 1 mg/mL Table S2: List of antibodies used for Western blot.

Review

10355 1 ap (Proteintech)

Structured Review

Images

1) Product Images from "Ghrelin Alleviates Aging‐Related Cognitive Impairment by Regulating Autophagy‐Related Signaling via the SHBG / JNK1 /Beclin1 Axis in Microglia"

Related Articles

Incubation:

Western Blot:

Blocking Assay:

other:

Immunohistochemistry:

Concentration Assay:

Data-independent acquisition:

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